International Journal of Research in Advanced Electronics Engineering
2024, Vol. 5, Issue 1, Part A
CRISPR-Cas based nano-sensors in water pathogen detection
Author(s): Shrestha Mukherjee, Subhasis Sarkar, Suranjana Sarkar, Semanti Ghosh and Bidisha Ghosh
Abstract: Waterborne diseases are primarily caused by various indiscriminate anthropological activities as a result of fast pace in industrialization coupled with the infestation of microorganisms borne as a result of the same. Water pollution thus is a major concern for public health in economically unprivileged nations. Assessing the water pathogens and monitoring quality is crucial for decision-making in water distribution systems, treatment, and prevention. Early detection of harmful microorganisms is essential for research, medical diagnosis, and public health. The polymerase chain reaction (PCR) approach has limitations due to expensive instrument control, unique test sites, and rigorous solution treatment steps. It may be possible to close detection gaps using the, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) -CAS system, which is a component of the bacterial adaptive immune system. Considering its capacity to target particular genes, CRISPR/Cas system has lately grown to favour as a diagnostic tool as functions in cleaving the target DNA or RNA using Cas enzymes and a guide RNA (gRNA). Nanotechnologies have shown promising applications in identifying, discriminating, and removing water pathogens. Nano-sensors, based on nanotechnology, can be utilized as a fruitful alarm to quickly detect pathogen contamination, for further improvement of water quality using nanoparticles like molecular-recognition probes. These are high strength materials for sensing applications, producing measurable signals. The CRISPR / Cas technology for pathogen detection combines good specificity, high sensitivity, and ease, making it appropriate for real-world applications. This review describes CRISPR/Cas-based nano-sensors for the detection of many dangerous water-borne pathogens, including Salmonella enteritidis, Salmonella species, and Pseudomonas species.
DOI: 10.22271/27084558.2024.v5.i1a.32
Pages: 07-10 | Views: 273 | Downloads: 182
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How to cite this article:
Shrestha Mukherjee, Subhasis Sarkar, Suranjana Sarkar, Semanti Ghosh, Bidisha Ghosh. CRISPR-Cas based nano-sensors in water pathogen detection. Int J Res Adv Electron Eng 2024;5(1):07-10. DOI: 10.22271/27084558.2024.v5.i1a.32
